This part of the Forsburg Lab website provides technical references about working with S. pombe. These general techniques papers describe practical applications, plasmids, markers, and the like. You might want to print out this list and put it in your notebook, as you will almost certainly need to reference some of these papers for your work on fission yeast.
Apolinario, E., Nocero, M., Jin, M. and Hoffman, C. S.
(1993). Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as
a new selectable marker for molecular genetic studies.
Curr. Genet. 24:491-495. Bach, M. L. (1987). Cloning and expression of the omp decarboxylase gene ura4 from Schizosaccharomyces pombe.
Curr. Genet. 12, 527-534.
Bähler J., Wu J.Q., Longtine M.S., Shah N.G., McKenzie A. 3rd, Steever A.B., Wach A., Philippsen P., Pringle J.R. (1998). Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe.
Yeast14: 943-951.
You can get the plasmids from Jurg Bähler or from Jianqiu Wu
Barbet, N., Muriel, W. J. and Carr, A. M. (1992). Versatile
shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe.
Gene 114:59-66. Basi, G., Schmid, E. and Maundrell, K. (1993). TATA box
mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription
efficiency but not the transcription start point or thiamine repressibility. Gene
123:131-136.
Beach, D., Piper, P. and Nurse, P. (1982). Construction of a
Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its
use to isolate genes by complementation. Mol. Gen. Genet. 187:326-329. Bonfils D, Gmunder H, Lehmann E, Rothlisberger M, Gafner J. (1989) Characterisation of an ARS-plasmid stabilisation element in the fission yeast Schizosaccharomyces pombe. Yeast. 1989 Apr;5 Spec No:S261-6.. The sequence of the fission yeast stb element.Brun, C., Dubey, D. D. and Huberman, J. A. (1995). Pdblet, a
Stable Autonomously Replicating Shuttle Vector For Schizosaccharomyces pombe.
Gene 164:173-177. Burke, J. D. and Gould, K. L. (1994). Molecular cloning and
characterization of the Schizosaccharomyces pombe his3+ gene for use as a
selectable marker. Mol Gen Genet 242:169-176. Burland, T. G., Pallotta, D., Tardif, M. C., Lemieux, G. and Dove, W. F. (1991).
Fission yeast promoter-probe vectors based on hygromycin resistance. Gene 100
241-245. Chang EC, Barr M, Wang Y, Jung V, Xu HP, Wigler MH (1994). Cooperative interaction of S. pombe proteins required for mating
and morphogenesis.
Cell 79: 131-141 Cottarel G, Beach D, and Deuschle U.(1993) Two new
multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2.
Curr. Genet. 23:547-8. Cottarel, G. (1995) The S. cerevisiae HIS3 and LYS2 genes
complement that S. pombe his5-303 and lys1-131 mutations, respectively: new
selectable markers and new multi-purpose multicopy shuttle vectors, pSP3 and
pSP4. Curr. Genet 28: 380-383
Craven, R.A., Griffiths, D.J., Sheldrick, K..S, Randall, R.E., Hagan, I.M., Carr, A.M. (1998) Vectors for the expression of tagged proteins in Schizosaccharomyces pombe.Gene 221:59-68 Ding DQ, Tomita Y, Yamamoto A, Chikashige Y, Haraguchi T, Hiraoka Y. (2000) Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library. Genes Cells. 5(3):169-190.
Ekwall, K and Ruusala, T. (1991) Budding yeast CAN1 gene as a
selection marker in fission yeast. Nucl. Acids Res. 19:1150 Faryar, K. and Gatz, C. (1992). Construction of a
tetracycline-inducible promoter in Schizosaccharomyces pombe. Curr Genet
21:345-349. Forsburg, S. L. (1993). Comparison of different
Schizosaccharomyces pombe expression systems.
Nucl. Acids Res. 21:2955-2956.Forsburg, S.L. (1994). Codon usage table for Schizosaccharomyces pombe.
Yeast 10: 1045-1047
Forsburg, S. L. (2001) The art and design of genetic screens: yeast. Nat. Rev. Genet. 2:659-668
Forsburg, S.L. (2003). Schizosaccharomyces pombe. In: Current Protocols in Molecular Biology, K. Struhl, section editor. New York: J. Wiley pp. 13.14.1-13.17.7.Forsburg, S.L. and Rhind, N. (2006) Basic methods for fission yeast. Yeast, 23:173-183Forsburg, S.L., and Sherman, D.A. (1997). General purpose tagging vectors for fission yeast. Gene 191: 191-195.
Grallert, B., Nurse, P., and Patterson, T. E. (1993). A study of integrative transformation in Schizosaccharomyces pombe.
Mol. Gen. Genet. 238, 26-32.
Grimm, C., Kohli, J., Murray, J., and Maundrell, K. (1988). Genetic engineering in Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.
Mol. Gen. Genet. 215, 81-86.
Grimm, C., BŠhler, J. and Kohli, J. (1994) M26 recombinational hotspot and physical conversion tract analysis in the ade6 gene of Schizosaccharomyces pombe. Genetics 135 41-51Hindley, J. Phear, G. , Stein, M. and Beach, D. (1987) suc1+
encodes a predicted 13-kd protein that is essential for cell viability and is
directly involved in the division cycle of S. pombe.
Mol. Cell. Biol. 7:504-511Hodson, J.A., Bailis, J.M, and Forsburg, S. L. (2003). Efficient labeling of fission yeast S. pombe by thymidine and BUdR. Nucl. Acids Res. 31:e134Hoffman, C. S. and Winston, F. (1991). Glucose repression of
transcription of the schizosaccharomyces pombe fbp1 gene occurs by a camp
signaling pathway. Genes Dev. 5:561-571.Javerzat, J.P., Cranston, G., Allshire, R.C. (1996). Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed. Nucl. Acids Res. 24: 4676-4683
Keeney, J. B. and Boeke, J. D. (1994). Efficient targeted
integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. .Genetics
136:849-856Kiely, J., Haase, S.B., Russell, P., and Leatherwood, J. (2000) Functions of fission yeast orp2+ in DNA replication and checkpoint Genetics 154:599-607.
Kikuchi, Y., Kitazawa, Y., Shimatake, H., and Yamamoto, M. (1988). The primary structure of the leu1+ gene of Schizosaccharomyces pombe.
Curr Genet 14, 375-9.
Losson, R., and Lacroute, F. (1983). Plasmids carrying the
yeast OMP decarboxy7lase structural and regulatory genes: Transcription
regulation in a foreign environment. Cell 32: 371-377. Maundrell, K., Hutchison, A., and Shall, S. (1988). Sequence analysis of ARS elements in fission yeast.
EMBO J. 7, 2203-2209.
Maundrell, K. (1990). nmt1 of fission yeast: a highly
transcribed gene completely repressed by thiamine. J. Biol. Chem.
265:10857-10864. Maundrell, K. (1993). Thiamine-repressible expression vectors
prep and prip for fission yeast. Gene 123:127-130. McLeod, M., Stein, M., and Beach, D. (1987) The product of the
mei3+ gene expressed under control of the mating type locus, induces meiosis and
sporulation in fission yeast. EMBO J. 6:729-736. McNabb, D.S., Pak, S.M., Guarente, L.P. (1997). Cassette for the generation of sequential gene disruptions in the yeast Schizosaccharomyces pombe. Biotechniques 22:1134-1139.Moreno, S., Klar, A., and Nurse, P. (1991). Molecular genetic analysis of the fission yeast Schizosaccharomyces pombe.
Methods Enzymol. 194, 795-823.
Moser, M.J., Lee, S.Y., Klevit, R.E., and Davis, T.N. (1995) Ca2+ binding to calmodulin and its role in Schizosaccharomyces pombe as revealed by mutagenesis and NMR spectroscopy. J. Biol. Chem. 270: 20643-52.
Nasim, A., Young, P.G., Johnson, B.F (Editors)
Molecular Biology of the Fission Yeast (Cell Biology Series) New York: Academic Press (1989)
Okazaki, K., Okazaki, N., Kume, K., Jinno, S., Tanaka, K. and Okayama, H. (1990). High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucl. Acids Res. 18:6485-6489.
Paluh, J.L., and Clayton, D.A. (1995) Schizosaccharomyces pombe RNase MRP RNA is homologous to metazoan RNase MRP RNAs and may provide clues to interrelationships between RNase MRP and RNase P. Yeast 11:1249-64.
Picard, D., Schena, M. and Yamamoto, K. R. (1990). An inducible
expression vector for both fission and budding yeast. Gene 86:257-261. Prabhala, G., Rosenberg, G. H., and Kaufer, N. F. (1992). Architectural features of pre-mRNA introns in the fission yeast Schizosaccharomyces pombe.
Yeast 8, 171-182.
Russell, P. R., and Hall, B. D. (1983). The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe.
J. Biol. Chem. 258, 143-149.
Russell, P. (1989). Gene cloning and expression in fission
yeast, in Molecular Biology of the fission yeast. Nasim, A., Young, P. and
Johnson, B. F., eds. Acadmic Press, San Diego. 244-272.
Schär, P. and Kohli, J. (1993) Marker effects of G to C transversions on intra- genic recombination and mismatch repair in Schizosaccharomyces pombe. Genetics 133 825-835.
Schär, P., Munz, P. and Kohli, J. (1993) Meiotic mismatch repair quantified on the basis of segregation patterns in Schizosaccharomyces pombe. Genetics 133 815-824Siam, R., Dolan, W.P., and Forsburg, S.L.(2004). Choosing and using S pombe plasmids. Methods 33:189-198
Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N. In vivo labeling of fission yeast DNA with thymidine and thymidine analogs.
Methods. 2004 33:213. Snaith HA, Samejima I, Sawin KE. Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast.
EMBO J. 2005 Nov 2;24(21):3690-9Szankasi, P., Heyer, W.-D., Schuchert, P., and Kohli, J. (1988). DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe.
J. Mol. Biol. 204, 917-925.
Tohda, H., Okada, H., Giga-Hama, Y., Okayama, H., and Kumagai, H. (1994) A copy-number controlled expression vector for the fission yeast Schizosaccharomyces pombe. Gene 150:275-280.
Waddell, S. and Jenkins, J.R.(1995) arg3+, a new selection
marker system for Schizosaccharomyces pombe: application of ura4+ as a removable
integration marker.
Nucl. Acids Res. 23: 1836-1837. Watt, S, Mata J, L—pez-Maury L, Marguerat S, Burns G, BŠhler J.
urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. (2008) PLoS ONE. 2008 Jan 16;3(1):e1428. Weilguny, D., Praetorius, M., Carr, A., Egel, R. and Nielsen, O.
(1991). New vectors in fission yeast: application for cloning the his2 gene.
Gene 99:47-54.
Zahn-Zabal M and Kohli J (1996) The distance dependence of the fission yeast ade6-M26 marekr effect in two-factor crosses.
Curr Genet 29:530-536
M.Q.Zhang and T.G.Marr (1994)
Fission yeast gene structure and recognition.
Nucl. Acid Res. 22: 1750
Zhao, Y., Goriparthi, L., Lieberman, H. B. (1994). A new shuttle vector system for the identification of spontaneous and radiation induced mutations in the fission yeast S. pombe. Mut. Res. 311:111-123.
Zhao, Y, Elder RT, Chen M, Cao J
(1998). Fission yeast expression vectors adapted for positive identification
of gene insertion and green fluorescent protein fusion. BioTechniques 25:438-443
© S. L. Forsburg .
